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KMID : 0895820150250020065
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Journal of Oriental Rehabilitation Medicine
2015 Volume.25 No. 2 p.65 ~ p.72
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Identification of Novel Standard Compounds for Standardization of Sinbaro3 Pharmacopuncture
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Lee Jin-Ho
Lee Jae-Woong
Kim Min-Jeong
Kim Eun-Ji
Lee In-Hee
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Abstract
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Objectives : To investigate and validate potential standard compounds for standardization of Sinbaro3 pharmacopuncture prepared at OO Hospital of Korean Medicine.
Methods : Sinbaro3 pharmacopuncture was prepared by extraction, purification and hydrolysis of Harpagophytum procumbens, and various potential standard compounds were quantified through HPLC-UV and HPLC-MS analysis. Validation was examined by assessing specificity, linearity, precision, and accuracy.
Results : The retention time of harpagide and cinnamic acid were 15.2 min and 28.2 min, respectively, and both showed good linearity in analysis by concentration at 0.9999 and 0.9998, respectively. Intra-day variation of precision was 0.0015~0.0045% and 0.0058~0.1629%, while inter-day variation of precision was 0.0011~0.0243% and 0.0098~0.1629%, and that of accuracy was 99.53~99.89% and 99.50~99.91%, respectively.
Conclusions : Harpagide and cinnamic acid, which are hydrolyzates of harpagoside within Sinbaro3 pharmacopuncture, were both validated using HPLC-MS and HPLC-UV analysis, and Sinbaro3 pharmacopuncture contained 78.41 ug/ml harpagide, and 2.05 ug/ml cinnamic acid.
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KEYWORD
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Shinbaro3, Harpagophytum procumbens, Standardization, Pharmacopuncture, HPLC-MS, Harpagide
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